Archaeologists and Aboriginal heritage officers have been removing sediment from eight trenches along the Jordan River levee at the Brighton roadworks site, north of Hobart.
Initial findings suggest the sediment is between 28,000 and 40,000 years old, making it the oldest, most southern site of human habitation in the world.
It is believed up to 3,000,000 artefacts could be buried there.
Dozens of protesters have been arrested and 19 people have been charged over protests aimed at trying to stop the roadwork in recent months.
Fiona Newson from the Tasmanian Aboriginal Land and Sea Council says the Tasmanian Goverment needs to take the latest report from archaeologists seriously.
“We’re talking about a worldwide significant site in regards to the scientific values and heritage values,” she said.
“It would be a total waste and not a good look on Tasmania if they were going to destroy it.”