It had been thought that Waldseemueller had only made four copies, but researchers at a Munich university have now discovered a fifth version.
This new map was found in the pages of an unrelated 19th century book.
Sven Kuttner, head of old books at Munich’s Ludwig Maximilian University, said: “It seems to be a second edition and this is a unique map. Until now, we have no signs for a further map like this.”
German researchers are going to make the map, printed in clear black ink on yellowing paper, available online from 4 July, Independence Day in the US.
Story: BBC News | Photo: AFP